Focus
Ancora biopsia? Forse no!
IS THE BIOPSY STILL NECESSARY? THE IMPORTANCE OF AUTOANTIBODIES AGAINST ACTIN FILAMENTS
S. De Virgilis
Settembre 2005 - pagg. 437 -455
Abstract
Recently, serum of celiac patients affected by autoimmune hepatitis revealed autoantibodies
directed against actin filaments (AAA). The preliminary results regarding AAA
showed a strong correlation between AAA antibody titre and the severity of intestinal damage.
The AAA testing showed high specificity given that AAA are not found in specific
conditions such as Crohns disease and autoimmune enteropathy. Further observations
showed that the actin content of enterocytes increases if gluten is introduced in the colture.
These data may suggest the possibility to avoid intestinal biopsy in case of AAA positivity,
hence modifying the whole diagnostic protocol. At the same time, they may suggest that tissue
transglutaminase lead to the development of gluten-dependent autoimmunity against
actin with following tissue damage.
Parole chiave
Classificazione MeSH
Bibliografia
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7. Kayalar C, Ord T, Testa MP, Zhong LT, Bredesen DE. Cleavage of actin by interleukin 1 beta-converting enzyme to reverse DNAse I inhibition. PNAS 1996;93:2234-8.
8. Tomasek JJ, Hallyday NL, Updike DL, et al. Gelatinase A activation is regulated by the organization of the polymerized actin cytoskeleton. J Biol Chem 1997;272:7482-7.
9. Chintala SK, Sawaya R, Aggarwal BB, et al. Induction of matrix metalloproteinase-9 requires a polymerized actin cytoskeleton in human malignant glioma cells. J Biol Chem 1998; 272:1354-51.
10. Clemente MG, De Virgilis S, Fasano A, et al. Gliadin induces a time dependent increase in the cellular content of polymerised actin filaments. Gut 2003;52:218-23.
2. Bardella MT, Trovato C, Cesana BM, et al. Serological markers for coeliac disease: is it time to change? Dig Liver Dis 2001;33:426-31.
3. Ciacci C, Cavallaro R, Della Valle N, et al. The use of serum tTG-Ab assay in patients on gluten-free diet as measure of dietetic compliance. Gastroenterology 2002;122:588.
4. Sbarbati A, Valletta E, Bertini M, et al. Gluten sensitivity and normal histology: is the intestinal mucosa really normal? Dig Liver Dis 2003;35:768-73.
5. Clemente MG, Musu MP, Frau F, et al. Immune reaction against the cytoskeleton in celiac disease. Gut 2000;47:520-6.
6. Varedi M, Ghahary A, Scott PG, Tredget EE. Cytoskeleton regulates expression of genes for transforming growth factor-beta and extracellular matrix proteins in dermal fibroblasts. J Cell Physiol 1997;172:192-9.
7. Kayalar C, Ord T, Testa MP, Zhong LT, Bredesen DE. Cleavage of actin by interleukin 1 beta-converting enzyme to reverse DNAse I inhibition. PNAS 1996;93:2234-8.
8. Tomasek JJ, Hallyday NL, Updike DL, et al. Gelatinase A activation is regulated by the organization of the polymerized actin cytoskeleton. J Biol Chem 1997;272:7482-7.
9. Chintala SK, Sawaya R, Aggarwal BB, et al. Induction of matrix metalloproteinase-9 requires a polymerized actin cytoskeleton in human malignant glioma cells. J Biol Chem 1998; 272:1354-51.
10. Clemente MG, De Virgilis S, Fasano A, et al. Gliadin induces a time dependent increase in the cellular content of polymerised actin filaments. Gut 2003;52:218-23.